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Acute and subacute toxicity of Artemisia asistica Nakai Aqua-acupuncture Solution (ANAS) were studied in ICR mice. In acute toxicity test, mice were injected intraperitoneally with single dose of 1 ×, 5 ×, 10 × ANAS, and toxicological responeses were observed for consecutive 14 days. Mortality, body weight changes, organ weight, and serum chemistry were performed. The mortality and body weight changes of mice treated with 1 × and 5 × ANA5 were not affected during the experimental periods. With the 10 × ANAS treatment, there were dead animals and changes of body weight, organ weight and serum biochemical values were observed during the experimental period. In subacute toxicity test, mice were injected intraperitoneally with doses of 1 ×, 10 × ANAS for 14 days. No difference was found between control and 1 × ANAS treated group in mortality, changes of body weight and organ weight, and serum biochemical values. However, Dead animals, changes of body weight and organ weight, and increased serum biochemical values were observed with 10 × ANAS treated groups. These results suggest that 1 × ANAS causes no toxicity in acute and subacute toxicity tests. However 10 × ANAS causes toxicity in both tests.
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